Two types of stratified (two-layer) granules (type 1, G-1； type 2, G-2) were observed in the neutrophils of lampreys, Lethenteron camtschaticum and L. kessleri. The G-1 consisted of chromophobic inner layer (L0) and outer layer (L1). On the other hand, the G-2 had chromatophilic L0 [eosinophilic and basophilic (metaazurophilic) in L. camtschaticum, basophilic (metaazurophilic) in L. kessleri] and chromophobic L1. Both lamprey species had same cytochemical characteristics. The L0 of G-1 showed positive reaction to alkaline phosphatase. Some lysozomal enzymes, such as acid phosphatase, β-glucuronidase, α-naphtyl acetate esterase and naphthol AS-D chloroacetate esterase were detected in the L0 of G-2. The L0 of G-2 was also Sudan black B positive. The neutrophils lacked α-naphtyl butyrate esterase and peroxidase.