Morphological and cytochemical characteristics of neutrophils from lampreys (Arctic lamprey Lethenteron camtschaticum and Siberian brook lamprey L.kessleri)
Journal of National Fisheries University Volume 69 Issue 2
Page 41-49
published_at 2021-02
Title
ヤツメウナギ類 (カワヤツメ, シベリアヤツメ) の好中球の形態学的および細胞化学的特徴
Morphological and cytochemical characteristics of neutrophils from lampreys (Arctic lamprey Lethenteron camtschaticum and Siberian brook lamprey L.kessleri)
Source Identifiers
[PISSN] 0370-9361
Creator Keywords
granule
neutrophil
lamprey
morphology
cytochemistry
Two types of stratified (two-layer) granules (type 1, G-1; type 2, G-2) were observed in the neutrophils of lampreys, Lethenteron camtschaticum and L. kessleri. The G-1 consisted of chromophobic inner layer (L0) and outer layer (L1). On the other hand, the G-2 had chromatophilic L0 [eosinophilic and basophilic (metaazurophilic) in L. camtschaticum, basophilic (metaazurophilic) in L. kessleri] and chromophobic L1. Both lamprey species had same cytochemical characteristics. The L0 of G-1 showed positive reaction to alkaline phosphatase. Some lysozomal enzymes, such as acid phosphatase, β-glucuronidase, α-naphtyl acetate esterase and naphthol AS-D chloroacetate esterase were detected in the L0 of G-2. The L0 of G-2 was also Sudan black B positive. The neutrophils lacked α-naphtyl butyrate esterase and peroxidase.
Languages
jpn
Resource Type
departmental bulletin paper
Publishers
National Fisheries University
Date Issued
2021-02
File Version
Version of Record
Access Rights
open access
Relations
[ISSN]0370-9361