Three types of granules, two extraordinary types and one inducible type, were observed in the neutrophils of red seabream Pagrus major infected with Vibrio harveyi. Both extraordinary granule types (eoG-1^{Vh}, eoG-2^{Vh}) had similar morphologies but different cytochemical characteristics to those of ordinary granules (oG-1, oG-2) from non-infected fish. The eoG-1^{Vh} showed chromophobic, simple morphology (without stratified structure), peroxidase positive and lack of lysozomal enzymes. The eoG-2^{Vh} was stratified granule with twolayer structure [inner eosinophilic layer (L0) and outer chromophobic layer (L1)]. Lysozomal enzymes [acid phosphatase, β-glucuronidase (β-Glu) and esterases] and peroxidase (PO) were localized in L0 and L1, respectively. Both extraordinary types were Sudan black B (SBB) negative. Spot formation, a curious phenomenon appeared in PO- stained oG-2 (positive L1 and negative L0), was not observed in eoG-2^{Vh}. Many L0 of eoβG-2^{Vh} were β-Glu positive. Contrastively, this enzyme activity was detected in a few L0 of oG-2. Inducible granule (iG^{Vh}) was consisted of two chromophobic layers (inner L0 and outer L1). The L0 of iG^{Vh} showed positive reaction to alkaline phosphatase, PO and SBB. Esterases were detected not only in the L0 of eoG-2^{Vh} but also in the L1 of iG^{Vh}.