Neutrophil Granules of Red Seabream Pagrus major Infected with Vibrio harveyi

Vibrio harveyiに感染させたマダイの好中球顆粒

Neutrophil Granules of Red Seabream Pagrus major Infected with Vibrio harveyi

Creators Kondo Masakazu

Creators Harada Muneyuki

Creators Yasumoto Shinya

Three types of granules, two extraordinary types and one inducible type, were observed in the neutrophils of red seabream Pagrus major infected with Vibrio harveyi. Both extraordinary granule types (eoG-1^{Vh}, eoG-2^{Vh}) had similar morphologies but different cytochemical characteristics to those of ordinary granules (oG-1, oG-2) from non-infected fish. The eoG-1^{Vh} showed chromophobic, simple morphology (without stratified structure), peroxidase positive and lack of lysozomal enzymes. The eoG-2^{Vh} was stratified granule with twolayer structure [inner eosinophilic layer (L0) and outer chromophobic layer (L1)]. Lysozomal enzymes [acid phosphatase, β-glucuronidase (β-Glu) and esterases] and peroxidase (PO) were localized in L0 and L1, respectively. Both extraordinary types were Sudan black B (SBB) negative. Spot formation, a curious phenomenon appeared in PO- stained oG-2 (positive L1 and negative L0), was not observed in eoG-2^{Vh}. Many L0 of eoβG-2^{Vh} were β-Glu positive. Contrastively, this enzyme activity was detected in a few L0 of oG-2. Inducible granule (iG^{Vh}) was consisted of two chromophobic layers (inner L0 and outer L1). The L0 of iG^{Vh} showed positive reaction to alkaline phosphatase, PO and SBB. Esterases were detected not only in the L0 of eoG-2^{Vh} but also in the L1 of iG^{Vh}.

[ISSN]0370-9361