Two types of stratified (two-layered) granules (type 1, G-1； type 2, G-2) were observed in the neutrophils of the inshore hagfish Eptatretus burgeri. The G-1 consisted of chromophobic inner layer (L0) and basophilic outer layer (L1). On the other hand, the G-2 had basophilic L0 and chromophobic L1. The L0 of G-1 showed positive reaction to α-naphtyl butyrate esterase.