Two types of non-phagocytic granulocytes, type B and C granulocytes were observed in peripheral blood of Japanese angelshark Squatina japonica (Squatinidae, Squatiniformes, Squalimorphii, Elasmobranchii). Type B granulocyte (GB) had three types of granules (GBG-A, -B and -C) in the cytoplasm. The GBG-A was coarse, round and consisted of three layers (L0, chromophobic inner； L1, eosinophilic middle； L2, chromophobic outer). The GBG-B had similar shape, size and structure of GBG-A except for the staining character of middle layer (basophilic). The GBG-C was round, small and made up of two layers: Amphophilic inner (L0) and outer (L1) layers. The L1 of GBG-A and/or GBG-B showed positive reaction to toluidine blue (TB), Sudan black B (SBB),α-naphtyl acetate esterase (α-NAE), α-naphtyl butyrate esterase (α-NBE) and naphthol AS-D chloroacetate esterase (NASDCAE). On the other hand, in the GBG-C, SBB, acid phosphatase (AcP), α-NAE and NASDCAE were detected in L0, α-NBE in L1 and TB in both L0 and L1. Type C granulocyte (GC) had one type of granules (GCG) which show two-layer structure: Eosinophilic inner (L0) and outer (L1) layers. Several enzymes (AcP, α-NBE, NASDCAE) were detected in L0. Both types of non-phagocytic granulocytes lacked alkaline phosphatase, β-glucuronidase and peroxidase.