Staining principle in the mixtures of acidic and basic dyes

Previously, we speculated on the staining principle of triacid staining solution (neutral mixture) containing a dye complex. The principle could be explained solely by the relationship between the stained object and the dye complex. We propose here to extend the staining principles of triacid stain to explain the staining principles of other dye mixtures such as May-Grünwald (MG), Giemsa and MG-Giemsa. In a mixture of acidic and basic dyes, the staining characteristics of the object were presumably determined by the degree of stainability (degree of acidophilicity or basophilicity) of the object, the degree of stainability of both dyes (degree of basophilicity of the acidic dye and degree of acidophilicity of the basic dye), and the ratio and concentration of each dye.