Two types of granulocytes were observed in peripheral blood of three species (banded houndshark Triakis scyllium, starspotted smooth-hound Mustelus manazo and spotless smooth-hound M. griseus) of Triakidae sharks (Carcharhiniformes, Galeomorphii, Elasmobranchii). The neutrophil of each shark was the only phagocytic granulocyte and had two types of granules (neutrophil granule, NG； type A, NG-A； type B, NG-B) with stratified structure. The NG-A was consisted of three layers: chromophobic inner layer (L0), eosinophilic middle layer (L1) with chromophobic outer layer (L2). A round eosinophilic particle was observed in L1 of some NG-A. The NG-B was round or oval, and both inner (L0) and outer (L1) layers of this granule was chromophobic. Several lysozomal enzymes were detected in L0 of NG-B. The L0 of T. scyllium NG-B was β-glucuronidase (β-Glu) positive, but acid phosphatase (AcP) negative. Conversely, the L0 of NG-B from M. manazo and M. griseus was β-Glu negative (AcP positive). The L2 of NG-A also alpha-naphtyl acetate esterase positive. The neutrophils lacked alkaline phosphatase and peroxidase. In this report, morphological and cytochemical similarities of neutrophils among elasmobranchs were discussed.