Journal of National Fisheries University

PISSN : 0370-9361

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Journal of National Fisheries University Volume 66 Issue 1
published_at 2017-11

Oxygen and Acid–Base Status of Hemolymph in the Pacific oyster Crassostrea gigas after Cannulation of the Adductor Muscle

Oxygen and Acid–Base Status of Hemolymph in the Pacific oyster Crassostrea gigas after Cannulation of the Adductor Muscle
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Descriptions
We examined hemolymph O_2 partial pressure, pH, total CO_2 content, CO_2 partial pressure and bicarbonate concentration in order to evaluate the acid–base balance of the Pacific oyster Crassostrea gigas after pretreatment of the adductor muscle by cannulation. The hemolymph O_2 and acid–base properties changed just after surgery. The temporary and significantly fluctuation of hemolymph properties disappeared at 1h after surgery in this study, and the O_2 and acid–base status was stable afterwards in normoxic conditions. The results in this study showed the possibility that sampling with a cannula can collect hemolymph as required. This sampling may be useful, when respiratory and endocrine function must be monitored in minimally disturbed animals without the effects of handling.
Creator Keywords
Crassostrea gigas
Pacific oyster
cannulation
hemolymph
acid–base balance
adductor muscle