Oxygen and Acid–Base Status of Hemolymph in the Pacific oyster Crassostrea gigas after Cannulation of the Adductor Muscle

Oxygen and Acid–Base Status of Hemolymph in the Pacific oyster Crassostrea gigas after Cannulation of the Adductor Muscle

Creators Handa Takeshi

Creators Araki Akira

Creators Yamamoto Ken-ichi

We examined hemolymph O_2 partial pressure, pH, total CO_2 content, CO_2 partial pressure and bicarbonate concentration in order to evaluate the acid–base balance of the Pacific oyster Crassostrea gigas after pretreatment of the adductor muscle by cannulation. The hemolymph O_2 and acid–base properties changed just after surgery. The temporary and significantly fluctuation of hemolymph properties disappeared at 1h after surgery in this study, and the O_2 and acid–base status was stable afterwards in normoxic conditions. The results in this study showed the possibility that sampling with a cannula can collect hemolymph as required. This sampling may be useful, when respiratory and endocrine function must be monitored in minimally disturbed animals without the effects of handling.

[ISSN]0370-9361