Four types of granulocytes, neutrophil, basophil, eosinophil and small eosinophilic granulocyte, were observed in peripheral blood of whip stingray Dasyatis akajei (Dasyatidae, Myliobatiformes, Batoidea, Elasmobranchii). The neutrophil is the only phagocytic granulocyte and had two types of chromophobic granules (βG: type A, βG-A； type B, ϐG-B) with core in the cytoplasm. The ϐG-A was long-elliptic shape or round (round or oval), and consisted eosinophilic core and chromophobic surrounding. The ϐG-B was round (round or oval), and both core and surrounding of this granule was chromophobic. Several lysozomal enzymes were detected in ϐG-B, but the positive site was different among enzymes: Acid phosphatase,α-naphtyl acetate esterase and naphthol AS-D chloroacetate esterase were detected in the core, whileα-naphtyl butyrate esterase was in the surrounding. The neutrophils lacked alkaline phosphatase, ϐ-glucuronidase and peroxidase. The core of ϐG-A showed positive reaction to hematoxylin stain (Mayer’s).