Journal of National Fisheries University

PISSN : 0370-9361

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Journal of National Fisheries University Volume 64 Issue 3
published_at 2016-03

Estimation of CO_2 partial pressure and bicarbonate concentration in the hemolymph of the noble scallop Mimachlamys nobilis

Estimation of CO_2 partial pressure and bicarbonate concentration in the hemolymph of the noble scallop Mimachlamys nobilis
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Descriptions
We examined hemolymph pH, total CO_2 content (Tco_2, mM/l), CO_2 partial pressure (Pco_2, mmHg) and bicarbonate concentration ([HCO_3^-], mM/l) in order to evaluate the acid-base balance of the noble scallop Mimachlamys nobilis in normoxic conditions. Hemolymph was collected anaerobically through a cannula after catheterization of the adductor muscle. The mean values of hemolymph pH and Tco_2 were 7.442 and 1.50 mM/l, respectively. Using the CO_2 solubility coefficient and apparent dissociation constant of carbonic acid determined in this study, Pco_2 and [HCO_3^-] were calculated as 1.55 mmHg and 1.44 mM/l, respectively. These values were in same range as those of the hemolymph of other marine bivalves of Mytiloida, Pterioida and Ostreoida.
Creator Keywords
Mimachlamys nobilis
cannulation
hemolymph
acid-base balance
Pco_2
dissociation constant of carbonic acid