Morphological and cytochemical characteristics of neutrophil in Carangid fishes, jackmackerel(Trachurus japonicus), greater amberjack(Seriola dumerili)and yellowtail amberjack(S. lalandi), were examined by light microscopy. Three types of granules, eosinophilic granule（αG), chromophobic granule(βG)and basophilic granule(γG)were observed in the neutrophils of these fish species. Multiple Romanowsky-type stain valuation revealed that αGs of these fish species were stained eosinophilic with May-Grünwald(MG), but not with Giemsa. Eosinophilic stain of the αG was disappeared by Giemsa after MG. The βGs were unstained by Romanowsky-type stain and peroxidase-positive. The γG of greater amberjack and yellowtail amberjack were stained light blue with Giemsa, but unstained with MG. On the other hand, the γGs of jack-mackerel were stained not only with Giemsa, but also with MG.