Journal of National Fisheries University

PISSN : 0370-9361

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Journal of National Fisheries University Volume 58 Issue 3
published_at 2010-03

Morphological and Cytochemical Characteristics of Neutrophil from African Lungfish, Protopterus annectens

アフリカハイギョProtopterus annectens好中球の形態学的および細胞化学的特徴
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Descriptions
Morphological and cytochemical characteristics of neutrophil from African lungfish, Protopterus annectens, were examined by light microscopy. The neutrophils were round to oval (35-40μm in diameter) and the nucleus round to lobule-shaped. Only one type of granule, panchromatophilic granule (PG) was observed in the neutrophil. The PG was round to rod-shaped and stained panchromatophilic according to staining conditions. The PG was eosinophilic or methylenophilic(methylene blue positive) with May-Gr?nwald (MG). And further, the PG showed reddish purple with Giemsa stain. This color tone may be due to the metachromatic reaction by azure B in Giemsa (metaazurophilic). This granule was acid phosphatase, α-naphtyl acetate esterase, α-naphtyl butyrate esterase, naphthol AS-D chloroacetate esterase, PAS (α-amylase digestion resistance) and toluidine blue (metachromatic) positive.
Creator Keywords
African lungfish
Protopterus annectens
Neutrophil
Morphology
Cytochemistry