Morphological and Cytochemical Characteristics of Neutrophil from African Lungfish, Protopterus annectens

アフリカハイギョProtopterus annectens好中球の形態学的および細胞化学的特徴

Morphological and Cytochemical Characteristics of Neutrophil from African Lungfish, Protopterus annectens

Creators Kondo Masakazu

Creators Takahashi Yukinori

Morphological and cytochemical characteristics of neutrophil from African lungfish, Protopterus annectens, were examined by light microscopy. The neutrophils were round to oval (35-40μm in diameter) and the nucleus round to lobule-shaped. Only one type of granule, panchromatophilic granule (PG) was observed in the neutrophil. The PG was round to rod-shaped and stained panchromatophilic according to staining conditions. The PG was eosinophilic or methylenophilic（methylene blue positive) with May-Gr?nwald (MG). And further, the PG showed reddish purple with Giemsa stain. This color tone may be due to the metachromatic reaction by azure B in Giemsa (metaazurophilic). This granule was acid phosphatase, α-naphtyl acetate esterase, α-naphtyl butyrate esterase, naphthol AS-D chloroacetate esterase, PAS (α-amylase digestion resistance) and toluidine blue (metachromatic) positive.

[ISSN]0370-9361