Morphological and cytochemical characteristics of neutrophils in the threadsail filefish Stephanolepis cirrhifer and puffers (grass puffer Takifugu niphobles, finepatterned puffer T. poecilonotus) were examined by light microscopy. Two types of granules, eosinophilic granule (αG) and chromophobic granule (βG) were observed in the neutrophils of these fish. Multiple Romanowsky-type stain valuation revealed that αGs of these fish neutrophils were not stained with Giemsa, but stained with May-Grünwald (MG). The βG was unstained by Romanowsky-type stain and peroxidase (PO) positive. In finepatterned puffer, nucleus of the neutrophils was also PO positive.