Staining characteristics of two types of monocyte minor granules (MmSG, MmLG) in monocyte from redlip mullet (Chelon haematocheilus) were examined by light microscopy. MmSG were stained orange with eosin Y (EY) and reddish purple with methylene blue (MB). Therefore, the color of MmSG (red) which stained with May-Grünwald・Giemsa (MGG) may be formed with both dyes. On the other hand, MmLG were not stained with EY and MB, but dark blue with azure B (AB). The color (dark red) of MmLG stained with MGG reappeared on the smear stained with the mixture of EY and AB.