This study examines the mechanical and time period aspects of fishing operations. To establish the technical guideline to evaluate the work load and its environmental factor under the fishing operation, previous paper clarified the characteristics of working environments of fishing boat. In consideration of these results, this paper examined the methods of motion and time study of fishing operation. The results of this study include development of methods of measurement and analysis of fishing operations, which are carried out with work line, and defining the processes with time passes. It could be understood to have the feature at the time required in each processes by analyzing the time to work in detail. In addition, it was considered to cause the time change by the fishing operations aspects, such as the fishing boat, fishing gears, and fishing operation itself.