We investigated the oxygen consumption and resistance for hypoxia in captivity of coral trout grouper Plectropomus leopardus. The fish with an average bodyweight 3 g consumed 515, 667 and 654 mg/kg/h of oxygen at water temperature 25, 27 and 30℃, respectively. On the other hand, one with an average bodyweights 5 g consumed 468 and 804 mg/kg/h at 25 and 30℃. The former groups recorded a decrease in oxygen consumption rate from 5 mg/L. The latter groups recorded from 4 mg/L. In a water temperature ranges from 25 to 30℃, 3 g fish began to die at dissolved oxygen levels of 0.99 to 1.06 mg/L (oxygen saturation of 15.4 to 16.0%), while at 0.73 to 1.15 mg/L (oxygen saturation of 11.0 to 18.8%), 5 g fish groups began to die. This is a significant result and gives a scientific impact on hypoxia for coral trout grouper aquaculture.

the purpose of this study is to clarify how Company A of fisheries processing and sales Company A in N City, Yamaguchi Prefecture overcame the constraints and challenges of sixth industrialization, such as the existing industrial structure and the limitations of the capabilities of economic entities that many management entities face. In conclusion, with the cooperation of the fishery cooperative in Company A's efforts to become a sixth industrial, the fact that each employee was able to make use of their experience and know-how to overcome the constraints and issues. This is thought to have led to the establishment of the sixth industrialization.

In this study, focused on the waste heat energy from seafood processing plant as research to promote the use of unutilized energy. The waste heat energy from seafood processing plant contains large amounts of the high temperature waste gas from the production of fish cake, and the high temperature wastewater from the production of boiled whitebaits and bonito flakes. If these unutilized waste gases and wastewater could be recovered and utilized as the high heat sources, it would lead to significant energy savings. It is assumed that seawater will be used as the low heat source. Seafood processing plants are usually located close to the sea for processing fisheries caught in the sea, and we recommend the use of seawater as the cooling heat source. Therefore, it is necessary to design systems in consideration of the effects on the ocean environment in the area where seawater is discharged, which may lead to significant modification of the ocean environment. If the optimum flow rate of low heat source at maximum net power is identified, the pump power for seawater can be reduced and the flow rate of seawater into the heat exchanger can be minimized as needed, which is expected to reduce the amount of seawater contamination and lead to lower maintenance costs for the heat exchanger. As a result of this study, it was found that the optimum flow rate of low heat source exists for maximum net power and maximum turbine output. The maximum net power was higher when the temperature difference between inlet and outlet of low heat source was higher, while the maximum turbine output was higher when the temperature difference between inlet and outlet of low heat source was lower.

The author has previously developed and published energy and resource flow models focusing on the post-collection process of marine debris. These models were designed to examine the technical and economic feasibility of establishing businesses with energy systems. One of the previous studies examined the economic feasibility of a microgrid system combining a styrene oil conversion device from drifted styrofoam and a cogeneration system (CGS). The current study focuses on the “biomass gasification furnace and methanol synthesis furnace” technology, which has a proven track record with woody biomass. If this methanol synthesis furnace could be applied to drifting marine debris, it would be an option for providing energy to coastal facilities. In this study, an energy flow model was developed that can easily calculate the energy flow of a methanol synthesis furnace, CGS, and fuel supply system using biomethanol obtained from coastal biomass such as marine debris. If only marine debris is taken into account, the amount of biomass will be insufficient. For this reason, discarded fish and food residues from fishing ports and fish processing plants were also evaluated. Furthermore, this biomethanol can be used together with waste cooking oil to synthesize biodiesel fuel (FAME), enabling various applications including marine fuel. Using this energy model, the potential for energy self-sufficiency for coastal fisheries facilities (fishing ports, fish processing plants, and fishing boats) was calculated. The calculation results show energy selfsufficiency as a function of changes in biomass volume. However, they also found it difficult to be self-sufficient in electricity, heat, and fuel at the same time. Additionally, an optimization model was developed to determine the optimal size of the CGS, demonstrating the potential to improve the energy self-sufficiency of coastal fishery facilities.

The monocytes of lampreys [Lethenteron camtschaticum (adult), L. reissneri (adult), L. hattai (adult, ammocoetes)] were round or oval, low nucleus/cytoplasm ratio, and have striated (two-layered) granules (MoG). The MoG consisted of a basophilic inner layer (L0) and a chromophobic outer layer (L1). The L0 were positive for acid phosphatase, α-naphthyl acetate esterase, naphthol AS-D chloroacetate esterase and Sudan black B. Also, the L0 showed orthochromatic (blue) with toluidine blue. However, ꞵ-glucuronidase, α-naphthyl butyrate esterase and peroxidase were not detected in the monocytes, which were negative for periodic acid Schiff reaction, alcian blue, Sudan III, and oil red O. Kenji Kiyono reported the two types of monocytes (as blood histocytes), real and dubious monocytes, from the blood of hagfish Eptatretus burgeri. He observed both monocyte types in grown-up hagfish, but only real monocytes in undeveloped (details not stated) hagfish. His real monocytes in undeveloped hagfish were speculated as the monocytes of lamprey (mistaking the specimen), and the real monocytes in grown-up hagfish as poorly stained neutrophils. The dubious monocytes of Kiyono were considered as the real monocytes of hagfish.

Artur Pappenheim reported a poor description of the morphology of blood neutrophils from lamprey (Lampetra planeri； adult and ammocoetes) and hagfish (Myxine glutinosa) in Folia Haematologica (volume 8, 1909). Here, we inferred on the morphological characteristics of cyclostome neutrophils observed by Pappenheim based on his descriptions and our previous reports. He recognized neutrophilic leukocytes (mature neutrophils) in lamprey (adult and ammocoetes) and hagfish, and neutrophilic myelocytes (immature neutrophils) in ammocoetes and hagfish. This means the existence of specific granules and azure granules. However, the specific granules were considered as an inner layer (L0) of the striated granule (NG2), and the azure granules outer (L1) layer of the striated granule (NG2). The specific granules (=L0 of NG2) would probably have been stained with triacid (purple), acid dyes, and May-Grünwald·Giemsa (MGG； purple), but not with methylgreen-pyronine (MP). The azure granules (=L1 of NG1a) would also have stained purple with MGG, but negative with triacid, acidic dyes and MP.

Identification of leukocytes on tissue sections is important to elucidate the mechanism of swim bladder lesions. To determine the best fixative solution for a koi carp swim bladder, the swim bladders were fixed in 10% formalin, Bouin's, MFAA and Davidson's solution. The swim bladders fixed in MFAA or Davidson's solution were severely detached and twisted, whereas those fixed in 10% formalin and Bouin's solution kept their external shape. However, the majority of the 10% formalin-fixed specimens showed detachment of the tunica interna from the tunica externa under the light microscope. Therefore, Bouin’s solution was determined to be the most suitable fixing solution for the swim bladder. Imprints (head kidney-touched slides glass were fixed with Bouin's solution) and tissue sections of head kidney fixed in Bouin’s solution were stained with Mayer’s hematoxylin and eosin (HE) and May-Grünwald·Giemsa (MGG), and the best staining method for leukocytes identification was investigated. In the HE-stained specimens, identification of leukocytes by staining was difficult. On the other hand, MGG-stained specimens could be identified by staining. Fixation with Bouin's solution and MGG staining was determined to be the most suitable method for leukocytes identification in the swim bladder.

我々は，安静状態におけるイガイMytilus coruscus のヘモリンパ液の酸塩基平衡を調べた。水温18°Cでのヘモリンパ液 pH は7.601±0.023（平均値±標準偏差）， 全炭酸含量（Tco_2）は1.59±0.06 mM/Lを示した。水温23°CではpH 7.568±0.031， Tco_2 1.54±0.09 mM/Lを示した。ヘモリンパ液の二酸化炭素分圧（Pco_2）と重炭酸イオン濃度（[HCO_3^–]）は，温度との関係式から推定された炭酸解離恒数（pKapp）を使用して計算された。Pco_2と[HCO_3^–] は水温18°Cで1.77±0.07 torrと1.50±0.06 mM/L，水温23°Cで1.83±0.08 torrと1.47±0.09 mM/Lを示した。推定したpKappを使い算出したPco_2を検証するため，本研究のin vitro実験で決定したpKappを用いてPco_2を計算した。異なる方法で算出した2つのPco_2に統計的な有意差は認められなかった。これらのことから，イガイヘモリンパ液のpKappを温度との関係式から推定することは，Pco_2と[HCO_3^–]の算出に有効と判断された。イガイヘモリンパ液の非重炭酸緩衝価（ꞵ_NB）は18°Cで0.42 slykes，23°Cで0.54 slykesであり，他のイガイ類の緩衝能をよく反映していた。

Characteristics of the feeding behavior and relationship between the standard length (SL) and the size of scrape marks of ayu Plecoglossus altivelis altivelis were studied using 123 cultured specimens in aquaria. Feeding behavior was observed individually, 116 specimens (94%) have mouth laterality for feeding. Also, feeding behavior were found two types. The first was eating the algae directly from the shelter in 91 specimens (78%). The second left the shelter and ate the algae after changing direction back to the shelter (22%). One side of the lower jaw when feeding on the acrylic plate side was turned downward and the other side of the jaws was closing. On the scrape marks, 11 to 14 and 11 to 13 level lines were visible caused by the upper and lower jaws and the algae was not scraped between the upper and lower parts. The comb–like teeth groups are arranged as 13 to 14 rows and 12 to 14 rows on the upper and lower jaws, respectively. Therefore, it is suggested that they use almost all of the comb–like teeth groups except the back teeth row on the upper and lower jaws for feeding. The lengths of upper and lower jaws were found to have power regression with the SL, respectively. Also, the relationship between the SL and full width of the scrape marks (FWSM) was： FWSM = 0.3939SL^0.6767 (R^2= 0.8392, 64-212 mm SL).

‘Hikoshima-haruna’ (Brassica rapa var. narinosa) is a local traditional vegetable in Shimonoseki (Yamaguchi, Japan). The mature leaves are edible and much of the harvest is processed into pickles for consumption in the Shimonoseki area. To expand the consumption of ‘Hikoshima-haruna’, we verified the use of ‘Hikoshima-haruna’ powder in fish paste products as a part of the utilization research. We prepared heated gels (Kamaboko) mixed with 0-10% ‘Hikoshima-haruna’ powder and measured their breaking strength. The Kamaboko containing 1.0-4.0% powder exhibited a bright green color, preserving the characteristics of ‘Hikoshima-haruna’ as a plant with bright green leaves. Although the breaking strength of the two-step heating (30℃ for 30 min followed by 85℃ for 20 min) gels tended to decrease with increasing powder concentration, the 1.0% powder concentration exhibited a slightly greater breaking strength than the other concentrations. Therefore, when applying ‘Hikoshima-haruna’ powder to fish paste products, its use as Kamaboko at a powder concentration of 1.0% was considered appropriate.

Previously, we speculated on the staining principle of triacid staining solution (neutral mixture) containing a dye complex. The principle could be explained solely by the relationship between the stained object and the dye complex. We propose here to extend the staining principles of triacid stain to explain the staining principles of other dye mixtures such as May-Grünwald (MG), Giemsa and MG-Giemsa. In a mixture of acidic and basic dyes, the staining characteristics of the object were presumably determined by the degree of stainability (degree of acidophilicity or basophilicity) of the object, the degree of stainability of both dyes (degree of basophilicity of the acidic dye and degree of acidophilicity of the basic dye), and the ratio and concentration of each dye.

Triacid staining solution (neutral mixture) contains dye complex. Here, we speculate on the structure and characteristics of the dye complex in various triacid staining solutions. It was inferred that the dye complexes in the Ehrlich (methyl green + 2 acid fuchsin, methyl green + 2 orange G, methyl green + acid fuchsin + orange G), Romanowsky (eosin + methylene blue), and Pappenheim (eosin + methylene azure) triacid stains behave as acidic dyes, and that the dye complexes do not dissociate against weakly acidophilic objects, resulting in staining with a complex color (purple). Therefore, it can be said that the objects to which the dye complexes bind without dissociation do not need to be basophilic. When the stained material is highly acidophilic or basic, the dye complex dissociates, and the acidophilic object is stained by the acidic dye and the basiophilic object by the basic dye. The dissociation of dye complexes depends on the degree of acidophilicity and basophilicity of the stained material, and the dissociation depends on the types of dye composing the dye complexes. The dye complex in a triacid stain can be defined as a purple acidic dye in which the bonds between the acidic and basic dyes in the complex can be broken, depending on the acidophilic and basophilic strength of the object.

In 2023, a new seafood cracker named Bakan-Senbei was commercialized in collaboration with National Fisheries University and two private companies. The standout feature of Bakan-Senbei is that it is made from low-utilized/unutilized fish, which contributes to the Sustainable Development Goals. Focusing on this feature, two types of questionnaires（A and B）were implemented to evaluate visitors’ perception of its quality at the Osaka Seafood Show held in February 2024. In questionnaire A, we investigated the potential demand price after providing product information. In questionnaire B, the potential demand price was examined by intentionally excluding only the material information, and then the potential demand price was examined while first excluding material information； then it was examined again after only providing material information and excluding lust time. Interval regression analysis revealed no significant difference between the potential demand prices from the two questionnaires. This result revealed that consumers did not recognize the added value converted into monetary value as perceived quality of the use of low-utilized/unutilized fish. This result indicated that consumers did not perceive the added value of using low-utilized /unutilized fish as translating into monetary value in terms of quality. Conversely, there was a significant difference between the two groups regarding the provision of material information in questionnaire B. This result was supported by the outcome that 21% of respondents raised the rank of potential demand price due to the provision of material information. We estimated the increase price to be 5.5 yen, which was 4.5% of the list price. It was suggested that the added value could be improved by providing important information.

イガイの呼吸機能，特に酸塩基平衡を評価するため，イガイ閉殻筋から採取したヘモリンパ液を用いて，二酸化炭素溶解度（αco_2）と炭酸解離恒数（pKapp）に及ぼす温度の影響について調査した。各実験温度において，イガイのヘモリンパ液を二酸化炭素標準ガスと平衡させ，pHと全炭酸含量を測定し，温度（T）とαco_2あるいはpKappとの関係を分析したところ，以下の関係式を得た。αco_2 = 138.247 – 11.253 • T + 0.554 • T^2 − 0.0140 • T^3 + 0.000138 • T^4，pKapp = 6.6407 − 0.01589 • T（αco_2： µM/L/torr； T： ℃）。これらの式により，任意の温度でイガイのヘモリンパ液におけるαco_2とpKappの推定が可能となった。これら推定値を用いれば，微量なヘモリンパ液の二酸化炭素分圧や炭酸水素イオン濃度を任意の温度で把握することができるだろう。

2025 年、第二次世界大戦後80 年を契機に周南公立大学（以下「本学」という。）経済経営学部の地域ゼミにおいて、周南市の戦中・戦後復興の動きについて調査活動を進め、同年末に発表会を行い、学生のプロポーザル（提言書）を周南市に手渡した。また、地域ゼミ所属の学生などが創設した「平和志向のまちづくりの会」の活動が、同年5 月に公益財団法人 山口きらめき財団（以下「財団」という。）の若者チャレンジ応援事業に採択され、発表会と同時に実施した周南市民への発信面を担った。これにより、学習活動と発信活動の二階建ての取組みとなり、結果として、学習活動で一定の成果が見られた。

2024 年6 月に周南公立大学に学生サークル「探検部」が設立された。本稿は探検部設立の経緯および趣旨、また今日までの山行を中心とした活動実績をまとめたものである。 既存の学生探検団体の多くは、その永い歴史を背景に活動様式や運営体制が一定程度確立されている。一方で、本学探検部（以下、探検部と記す）は未経験者が集まった新設の学生団体であることから、活動内容や参加形態に柔軟性をもたせた運営を行っている。 山登りを軸としながらも、初心者が参加しやすい活動や、参加者の関心に応じた内容調整を行うことで、探検活動への参加のハードルを下げることを意識している点が本サークルの特徴である。 紙数の多くは、サークルの中心的な活動として実施した計５回の山行についてのそれぞれ紀行文形式での報告である。５回の山行とは、右田ヶ岳、三倉岳、三瓶山縦走、三鈷峰（大山）、比婆山連峰半縦走であり、いずれも日帰りないしは前泊日帰りのものであった。

本事例報告では、周南市において導入・展開されている学生消防団員制度及び、周南公立大学、徳山工業高等専門学校、周南市の三者連携による制度形成の経緯とその意義を示す。とりわけ、地域防災力の向上および若年層の地域参画という観点から、学生消防団制度が果たす役割について検討する。周南市では、平成29 年度に導入された「学生消防団活動認証制度」をもとにして、平成30 年3 月に学生消防団を創設した。その後に発生した新型コロナウイルス感染症の影響により一時的に団員数が減少し、2025 年3 月時点では学生消防団員は1 名となっていた。そこで、周南公立大学、徳山工業高等専門学校、周南市の三者連携に基づき新たな学生消防団を創設することとなった。以降、再び学生の参加が拡大しており、地域防災体制の持続可能性を支える重要な取り組みとなっている。

本研究は、駆け出し期の教職課程担当教員が抱えるゲートキーピング上の葛藤に着目し、その省察と言語化を支援するプラクティカムの可能性を検討するものである。教職課程科目では、多様な専門性やキャリアを有する教員が授業を担当する一方、授業設計に関する意思決定を省察的に捉える機会は限られている。そこで本研究では、大学1 年次向け「教師論」において、2 名の教員が役割分担制で授業を共同運営し、事前検討会と事後省察を組み込んだプログラムを試行した。その結果、初任期教員の授業構想や教材選択における判断が次第に精緻化されるとともに、協働的省察の相互作用が確認された。本研究は、教職課程担当教員の専門性開発に資する実践的示唆を提供する。