Neutrophils observed in the blood of red seabream Pagrus major with parasitized Ceratothoa verrucosa were characterized by the inducible chromophobic granules (iβG^{Cv}) with two-layer structure (inner chromophobic layer (L0) and outer eosinophilic layer (L1)). A curious phenomenon, spot formation, was observed in/above the May-Grünwald ・ Giemsa-stained iβG^{Cv}. Dark brown and round spot was overlapped in L0 of iβG^{Cv}. Neutrophil was only granulocyte type and almost all neutrophils had the iβG^{Cv} in the head kidney (HK； hematopoietic tissue) of infected fish. On the other hand, two types of granulocytes, a few iβG^{Cv} -containing neutrophils and some large granulocytes, were observed in the spleen of infected fish. The latter was large-sized round or ovoid cell with eccentric nucleus and chromophobic large round granules. In the non-infected fish, neutrophils in blood, HK and spleen lacked iβG^{Cv}, and large granulocytes were not found in the spleen.