On the basis of our new observations, we propose the structure of granules in neutrophil and eosinophil of whip stingray Dasyatis akajei. The neutrophil granules (NG) were classified into two types (type A, NG-A； type B, NG-B). The NG-A consisted of eosinophilic granule core (layer 0 + layer 1) and chromophobic surrounding of core (mantle； layer 2). The core had stratified structure: Eosinophilic inner (layer 0) and outer core (layer 1). The NG-B was made up of chromophobic granule core (layer 0) and mantle (layer 1). The eosinophil had two types of granules, eosinophil granules (EG； type A, EG-A； type B, EG-B). The EG-A consisted of granule core (layer 0 + layer 1) and chromophobic mantle (layer 2). The core had stratified structure: Chromophobic inner core (layer 0) and eosinophilic outer core (layer 1). The EG-B was chromophobic and non-stratified granule. The outer cores (layer 1) of NG-A and EG-A were stained with hematoxylin (Mayer).