Morphological and cytochemical characteristics of neutrophil from African lungfish, Protopterus annectens, were examined by light microscopy. The neutrophils were round to oval (35-40μm in diameter) and the nucleus round to lobule-shaped. Only one type of granule, panchromatophilic granule (PG) was observed in the neutrophil. The PG was round to rod-shaped and stained panchromatophilic according to staining conditions. The PG was eosinophilic or methylenophilic（methylene blue positive) with May-Gr?nwald (MG). And further, the PG showed reddish purple with Giemsa stain. This color tone may be due to the metachromatic reaction by azure B in Giemsa (metaazurophilic). This granule was acid phosphatase, α-naphtyl acetate esterase, α-naphtyl butyrate esterase, naphthol AS-D chloroacetate esterase, PAS (α-amylase digestion resistance) and toluidine blue (metachromatic) positive.