コンテンツメニュー

Yamasaki Yasuhiro


Growth inhibition of the haptophyte Diacronema lutheri by self-growth inhibitors

Journal of National Fisheries University Volume 63 Issue 3 Page 209-218
published_at 2015-03
63-3-209-218.pdf
[fulltext] 681 KB
Title
Growth inhibition of the haptophyte Diacronema lutheri by self-growth inhibitors
Creators Yamasaki Yasuhiro
Creators Mii Shunsaku
Creators Mitani Takashi
Source Identifiers [PISSN] 0370-9361
Creator Keywords
Allelopathy Algal diet Growth curves Haptophyte Diacronema lutheri Self-growth inhibition
Better understanding of the factors that affect the growth of microalgae used as feed in the aquaculture industry will help to improve cultivation methods. In this study, we investigated the effects of Diacronema lutheri filtrates on the growth of D. lutheri itself under various growth conditions to determine whether allelochemicals were affecting the growth of this dietary alga. Our results indicated that filtrates of the D. lutheri reduced the maximum fluorescence of D. lutheri cells in a concentrationdependent manner under various test conditions. In addition, growth inhibitors that accumulated at high concentrations in the culture medium inhibited the growth of D. lutheri when the cultures were incubated at a high temperature. On the other hand, the inhibitory effect of D. lutheri filtrate on the growth of D. lutheri was a species-specific phenomenon, because growth of D. lutheri was not affected by Nannochloropsis sp. filtrate. Furthermore, the permeate fraction obtained by ultrafiltration significantly inhibited the growth of D. lutheri(P < 0.05), but the retentate fraction did not. These results suggest that lowmolecular- weight substances, with the exception of proteins, are involved in the inhibitory effect of D. lutheri filtrate, and developing a process for removal of growth-inhibitors from culture media will contribute to improving the D. lutheri mass culture methodology at high water temperatures.
Languages eng
Resource Type departmental bulletin paper
Publishers National Fisheries University
Date Issued 2015-03
File Version Version of Record
Access Rights open access
Relations
[ISSN]0370-9361