Only one type of non-phagocytic granulocyte (type B granulocyte, GB) was observed in peripheral blood of three Triakidae sharks (banded houndshark Triakis scyllium, starspotted smooth-hound Mustelus manazo and spotless smooth-hound M. griseus； Carcharhiniformes, Galeomorphii, Elasmobranchii). The GB had two types of granules (GBG； type a, GBG-a; type b, GBG-b). The GBG-a was coarse without stratified structure, round or oval (subangular to angular) in shape and chromophobic. The GBG-b was smaller than GBG-a, round or oval with stratified structure (inner L0 and outer L1) and chromophobic. Beta-glucuronidase activity was detected in L0 of GBG-b from T. scyllium (negative in Mustelus sharks), and acid phosphatase was localized in L0 of GBG-b from Mustelus sharks (negative in T. scyllium). Localization of α-naphtyl acetate esterase and α-naphtyl butyrate esterase were different among Triakidae sharks. The GB lacked alkaline phosphatase and peroxidase. In this report, morphological and cytochemical differences of non-phagocytic granulocytes among elasmobranchs were discussed.