Numerous alkaline phosphatase (AIP)-positive granules appeared in neutrophils of red seabream Pagrus major after infection with pathogenic bacteria., atypical Edwardsiella tarda (=Edwardsiella anguillarum). This granule consisted of AIP-positive core and its AIP-negative surrounding. Both parts were chromophobic with May-Grunwald ・ Giemsa stain, and react negatively to several lysosomal enzymes, peroxidase, Sudan black B, etc. This granule type was not found in the neutrophils from non-infected fish. Therefore, the granules may be induced by infection with E. tarda. We designate the granule inducible chromophobic granules (iβG).