We investigated the effect on growth and maturation of gametophyte in Eisenia bicyclis at 20℃, 12 h light- 12 h dark cycle and 50μmol m^{-2} s^{-1} using four different light emitting diodes（LEDs）and a fluorescent light . The relative growth rates of the male and female gametophytes under blue LEDs were obtained high values, but that under red LEDs were obtained low values. The maturation of the female gametophyte was promoted under white and blue LEDs, and was inhibited under red LEDs. Thus, it were clarified that the growth and maturation of gametophyte in Eisenia bicyclis were promoted by using white and blue LEDs, and were inhibited by using red LEDs.