Ｍorphological and cytochemical characteristics of neutrophil in hagfish, Eptatretus burgeri were examined by light microscopy and the composition of neutrophil granule were inferred in this study. The neutrophils were round or oval (15-20μm in diameter) and the nucleus round to two lobule-shaped. Only one types of granule ； basophilic granule (γG) was observed in the neutrophil. The γG was round or oval (≦0.5μm in diameter) and stained with not only MG using distilled water and 5 mM phosphate buffer, but also Giemsa and MGG. Alpha-naphthyl butyrate esterase was detected in the γG. Yasumoto body (Y-body) was also observed in neutrophil and stained with toluidine blue.