The aim of this study is to examine the reliability and validity of the Competency Scale in Practice of Childhood Care for children with Disabilities (CSPCD). Moreover, this study examines the relationship between CPSCD and careers of nursery teachers. The results exhibited that CSPCD consisted of five sub-scales interest in childcare for children with disabilities necessary assessments in childcare for children with disabilities; the actual involvement in childcare for children with disabilities; collaboration with parents and other organizations in childcare for children with disabilities, and development of expertise in childcare. Our study finds that there is sufficient reliability and validity in this regard due to significant correlation between the score of interest in childcare for children with disabilities in CSPCD and the required number of years of experience for a childcare institution. Additionally, it was clarified that the total score of CSPCD and the scores of each sub-scale differed significantly depending on the teachers’ individual experiences in assisting children with disabilities.