Estimation of hemolymph αco_2 and pKapp in disk abalone Haliotis (Nordotis) discus discus between 10℃ and 20℃

クロアワビのヘモリンパ液における二酸化炭素溶解度と炭酸解離恒数の推定

Estimation of hemolymph αco_2 and pKapp in disk abalone Haliotis (Nordotis) discus discus between 10℃ and 20℃

We investigated the effect of temperature on disk abalone Haliotis (Nordotis) discus discus hemolymph CO_2 solubility coefficient (αco_2) and the apparent dissociation constant of carbonic acid (pKapp). The disk abalone hemolymph was equilibrated with a standard CO_2 gas mixture between 10℃ and 20℃, to obtain expressions for αco_2 and pKapp as a function of temperature. The relationship between αco_2 and temperature (T) is expressed as follows： αco_2 = 74.005 - 1.2936 • T - 0.00944 • T^2. And the relationship between pKapp and temperature expressed as follows： pKapp = 6.4675 - 0.08682 • T + 0.003996 • T^2. In these equations, the parameter units are ℃ for T and µM/L/torr for αco_2. The non-bicarbonate buffer values (ꞵ_NB), obtained as a regression coefficient relating pH and [HCO_3^–], were 2.5 Slykes at 10℃, 2.2 Slykes at 15℃, and 3.4 Slykes at 20℃. These equations enable estimation of hemolymph αco_2 and pKapp between temperatures of 10℃ and 20℃.

Creators Handa Takeshi

Creators Araki Akira

[ISSN]0370-9361