タイノエに寄生されたマダイの頭腎と脾臓に観察される顆粒球について

On the granulocytes observed in head kidney and spleen of red seabream Pagrus major with parasitized Ceratothoa verrucosa

Creators Kondo Masakazu

Creators Yasumoto Shinya

Creators Takahashi Yukinori

Neutrophils observed in the blood of red seabream Pagrus major with parasitized Ceratothoa verrucosa were characterized by the inducible chromophobic granules (iβG^{Cv}) with two-layer structure (inner chromophobic layer (L0) and outer eosinophilic layer (L1)). A curious phenomenon, spot formation, was observed in/above the May-Grünwald ・ Giemsa-stained iβG^{Cv}. Dark brown and round spot was overlapped in L0 of iβG^{Cv}. Neutrophil was only granulocyte type and almost all neutrophils had the iβG^{Cv} in the head kidney (HK； hematopoietic tissue) of infected fish. On the other hand, two types of granulocytes, a few iβG^{Cv} -containing neutrophils and some large granulocytes, were observed in the spleen of infected fish. The latter was large-sized round or ovoid cell with eccentric nucleus and chromophobic large round granules. In the non-infected fish, neutrophils in blood, HK and spleen lacked iβG^{Cv}, and large granulocytes were not found in the spleen.

[ISSN]0370-9361