滑走細菌Tenacibaculum maritimum に感染させたマダイの好中球顆粒

Neutrophil granules of red seabream Pagrus major infected with gliding bacterium Tenacibaculum maritimum

Creators Kondo Masakazu

Creators Watanabe Riho

Creators Tachibanagi Keito

Creators Kimura Takehiro

Creators Yasumoto Shinya

Three types of granules, two extraordinary types and one inducible type, were observed in the neutrophils of red seabream Pagrus major infected with Tenacibaculum maritimum. Both extraordinary granule types (eoG1^{Tm}, eoG2^{Tm}) had similar morphologies but different cytochemical characteristics to those of ordinary granules (oG1^N, oG2^N) from non-infected fish. The eoG1^{Tm} showed chromophobic, simple morphology (without stratified structure), peroxidase positive and lack of lysozomal enzymes. The eoG2^{Tm} was stratified granule with three-layer structure [inner eosinophilic layer (L0-0), middle chromophobic layer (L0-1) and outer chromophobic layer (L1)]. Acid phosphatase, ꞵ-glucuronidase (ꞵ-Glu), α-naphtyl acetate esterase and naphthol AS-D chloroacetate esterase were detected in L0-0, but α-naphtyl butylate esterase was in L0 (L0-0 and L0-1). Peroxidase (PO) were localized in L1. Both extraordinary types were Sudan black B (SBB) negative. Spot formation, a curious phenomenon appeared in PO-stained oG2^N (positive L1 and negative L0), was not observed in eoG2^{Tm}. Inducible granule (iG^{Tm}) was consisted of two chromophobic layers (inner L0 and outer L1). The L0 of iG^{Tm} showed positive reaction to PO.

[ISSN]0370-9361