ラメロジスカス症に罹患したマダイの好中球顆粒

Neutrophil Granules of Red Seabream Pagrus major Infected with Lamellodiscosis

Creators Kondo Masakazu

Creators Yasumoto Shinya

Two types of chromophobic granules [type 1, ordinary type (oβG-1^{Ld})； type 2, extraordinary type (eoβG-2^{Ld})] were observed in the neutrophils of red seabream Pagrus major infected with Lamellodiscus spp. The oβG-1^{Ld} showed similar morphology and cytochemistry to type 1 ordinary chromophobic granule (oβG-1) from non-infected fish: Chromophobic, simple morphology (without stratified structure), positive reaction to peroxidase (PO) and Sudan black B (SBB), and lack of lysozomal enzymes. The eoβG-2^{Ld} was stratified granule with two-layer structure [inner eosinophilic layer (L0) and outer chromophobic layer (L1)]. Several lysozomal enzymes [acid phosphatase (AcP), β-glucuronidase, α-naphtyl acetate esterase, α-naphtyl butyrate esterase, naphthol AS-D chloroacetate exterase (CAE)] were detected in the L0, however, AcP and CAE were localized in the small central area of L0. These findings indicate that the L0 of eoβG-2^{Ld} consists of L0-0 (inner part in L0； AcP and CAE positive) and L0-1 (outer part of L0-0). The L1 showed positive reaction to SBB and PO. Spot formation, a curious phenomenon appeared in PO-stained type 2 ordinary chromophobic granule (oβG-2； positive L1 and negative L0) from non-infected fish, was not observed in eoβG-2^{Ld}.

[ISSN]0370-9361