Kanamaru Shunsuke
Morphological and Cytochemical Characteristics of Neutrophil from Japanese Amberjack, Seriola quinqueradiata
Journal of National Fisheries University Volume 58 Issue 2
Page 101-111
published_at 2009-12
Title
ブリの好中球の形態学的および細胞化学的特徴
Morphological and Cytochemical Characteristics of Neutrophil from Japanese Amberjack, Seriola quinqueradiata
Source Identifiers
[PISSN] 0370-9361
Creator Keywords
neutrophil
Japanese amberjack
Seriola quinqueradiata
morphology
Morphological and cytochemical characteristics of neutrophil in Japanese amberjack, Seriola quinqueradiata were examined by light microscopy. The neutrophils were round to oval (7.0-11.0μm in diameter) and the nucleus round to lobule-shaped. Granules of the neutrophil were classified into three types; acidophilic granule (αG), chromophobic granule (βG), chromophobic granule (βG) and basophilic granule (γG). The αG was rod-shaped (0.5-1.0μm in length, 0.2μm in width) and stained with May-Grünwald (MG) stain. This granule was not observed in the preparations stained with Giemsa stain. The MG-Giemsa (MGG) staining pattern of the granule was influenced by pH and concentation of diluent of the staining solution. The βG was round to oval (≦0.5μm in diameter) and unstained by Romanovsky type stain (MG, Giemsa and MGG). This granule was alkaline phosphatase, peroxidase and sudan black B positive. The γG was round to oval (≦0.3μm in diameter) and stained with Giemsa and MGG, but not with MG. This granule was α-naphtyl acetate esterase positive. The Yasumoto body (Y-body) was also found in the neutrophil and toluidine blue positive.
Languages
jpn
Resource Type
departmental bulletin paper
Publishers
National Fisheries University
Date Issued
2009-12
File Version
Version of Record
Access Rights
open access
Relations
[ISSN]0370-9361