Characterization of triacid staining solution containing free acidic dyes

遊離した酸性色素を含むtriacid染色液の特徴

Characterization of triacid staining solution containing free acidic dyes

Triacid staining is a technique used to confirm the neutrophilic properties of constituents, resulting in a purple color in neutrophilic substances such as neutrophilic granules. However, it is known that neutrophilic substances that are normally purple in triacid staining may occasionally exhibit the color of the acidic dye, which is one of the dyes included in the triacid staining solution. This study reports that the cause is the presence of excessive free acidic dye in the triacid staining solution and examines this staining model. The dye complex formed in a triacid staining solution consisting of orange G, acid fuchsin, and methyl green has two binding sites for neutrophilic substances, and free acidic dyes may be mixed with this complex. In such cases, it was inferred that the preferential binding of the acidic dye to the neutrophilic substance was due to the number of acidic groups corresponding to the free acidic groups of the acidic dye being higher than the number of free acidic groups at the binding sites of the dye complex.

Creators Kondo Masakazu

Creators Yasumoto Shinya

[ISSN]0370-9361