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Morphological and cytochemical characteristics of neutrophils from adult of far eastern brook lamprey Lethenteron reissneri

Journal of National Fisheries University Volume 71 Issue 4 Page 109-122
published_at 2023-03
71-4-109-122.pdf
[fulltext] 2.19 MB
Title
スナヤツメ成魚の好中球の形態学的および細胞化学的特徴
Morphological and cytochemical characteristics of neutrophils from adult of far eastern brook lamprey Lethenteron reissneri
Abstract
Neutrophil granules (NG) of adult (mature) far eastern brook lamprey Lethenteron reissneri [L. sp. S (souther form)] were classified into two types of stratified [two-layer; inner layer (L0) and outer layer (L1)] granules (type 1, NG1a; type 2, NG2), like as larva (ammocoetes) of this species. The L0 of NG1a and L0 of NG2 in adult neutrophils were chromophobic, and L1 of NG1a and L0 of NG2 showed chromatophilic. The stainability of those chromatophilic layers with May-Grünwald (MG), Giemsa, or MG-Giemsa were varied [chromophobic, eosinophilic or basophilic (orthomethylenophilic or metaazurophilic)] with a slight difference to larva. As seen in larval neutrophils, these layers were positive for alkaline phosphatase,α-naphtyl acetate esterase and Sudan black B, and some enzymes (acid phosphatase, ꞵ-glucuronidase, and naphthol AS-D chloroacetate esterase) were detected in the L0 of NG2. Alpha-naphtyl acetate esterase was positive in L0 of NG2 of adult neutrophils. Both larval and adult neutrophils lacked peroxidase.
Creators Kondo Masakazu
Creators Yasumoto Shinya
Source Identifiers [PISSN] 0370-9361
Creator Keywords
lamprey Lethenteron reissneri neutrophil morphology cytochemistry
Languages jpn
Resource Type departmental bulletin paper
Publishers National Fisheries University
Date Issued 2023-03
File Version Version of Record
Access Rights open access
Relations
[ISSN]0370-9361