The purpose of this study was to examine the impact of using posters to provide blood donation information on university students’ willingness to donate blood. The participants were 175 nursing students (mean age 19.42 ± 1.13 years) at University A in Yamaguchi Prefecture. Posters displaying blood donation information and the blood inventory status published by the Japanese Red Cross Society were placed inside elevators for two weeks. Subsequently, a questionnaire survey was conducted. Of the 175 students, 29 (16.6%) had previous blood donation experience, and 146 (83.4%) had none; 72 (41.1%) had attended a lecture on blood donation, while 103 (58.9%) had not. Both experiences were significantly less common. During the survey period, 122 students (69.7%) used the elevator, and among them, 64 (52.5%) viewed the posters. Among those with blood donation experience and those with lecture attendance experience, a higher proportion had viewed the poster. This suggests that individuals with blood donation or lecture attendance experience may be more likely to pay attention to blood donation information. Factor analysis (maximum likelihood method with Promax rotation) of 13 blood donation information items identified three factors: “Understanding of Knowledge and Systems,” “Blood Donation Process and Precautions,” and “Blood Donation Service Information.” Multiple regression analysis, with “intention to donate blood within the next six months” as the dependent variable and “academic year,” “blood donation experience,” “lecture attendance,” and the three factors as independent variables, showed that blood donation intention was significantly associated with “blood donation experience,” “understanding of knowledge and systems,” and “blood donation process and precautions” (adjusted R² = .34), whereas “academic year,” “lecture attendance,” and “blood donation service information” were not significant. Furthermore, content analysis of reasons why poster displays increased interest revealed that visualization of blood type shortages and real-time information enhanced the perceived necessity of donation. These findings suggest that early and continuous education on blood donation, combined with information that helps individuals perceive donation as personally relevant, may be effective in promoting blood donation behavior.