Production of artificial malignant cells and their evaluation

人工的悪性細胞の作製とその評価について

Production of artificial malignant cells and their evaluation

Creators Kobayashi Wakako

Creators Gankhuyag Enkhjavkhlan

Creators Nakao Hikari

The transcription factor Snail is involved in gastrula invasion, organogenesis, and cancer cell invasion/ metastasis during early embryogenesis. Snail also has the function of inducing epithelial-mesenchymal transition (EMT). The author introduced a Snail gene expression vector into human colon cancer cell line DLD-1 cells to prepare Snail overexpression cells (called DLD-1/Snail cells). DLD-1/Snail cells changed from epithelial-like morphology to mesenchymal cell morphology and decreased proteins levels such as epithelial makers and increased migration and invasion, inducing EMT. As a result of microarray analysis, the expression of α-1,3/4 fucosyltransferase (Fuc-TⅢ) was decreased in DLD-1/Snail cells compared with control cells. Fuc-TⅢ is a glycosyltransferase required to produce the cancer-related chain antigen sialyl-lewis a (sLea). It was a ligand that adhesive to vascular endothelial cells via E-selectin. It has been suggested that sLea is involved in cancer cell metastasis. However, decreased expression of Fuc-TⅢ in DLD-1/Snail cells reduced sLea antigens. The above results found that during EMT induction by Snail, the cells suppressed the fucosyltransferase expression and eliminated the cell surface's sLea antigen.

[ISSN]0386-4359

[NCID]AN00019833