Morphological and cytochemical characteristics of neutrophil granules from West African lungfish Protopterus annectens

ニシアフリカハイギョの好中球顆粒の形態学的および細胞化学的特徴

Morphological and cytochemical characteristics of neutrophil granules from West African lungfish Protopterus annectens

Neutrophil granules (NG) of West African lungfish Protopterus annectens were classified into two types of stratified [two-layer; inner layer (L0) and outer layer (L1)] granules (type A, NG-A; type B, NG-B). The L1 of NG-A and both layers of NG-B were chromophobic, and L0 of NG-A showed polychromatophilic [eosinophilic or basophilic (orthomethylenophilic or metaazurophilic)]. The L0 of NG-A showed metachromatic (reddish purple) with toluidine blue. On the other hand, L0 of NG-B were positive for acid phosphatase, α-naphthyl acetate esterase, α-naphthyl butyrate esterase and naphthol AS-D chloroacetate esterase. Both types of granules were negative in periodic acid Schiff reaction, alcian blue, Sudan black B, Sudan III, and oil red O. Alkaline phosphatase, β-glucuronidase, and peroxidase were not detected in either granule.

Creators Kondo Masakazu

Creators Yasumoto Shinya

[ISSN]0370-9361