Morphological and cytochemical characteristics of neutrophils from lampreys (Arctic lamprey Lethenteron camtschaticum and Siberian brook lamprey L.kessleri)

ヤツメウナギ類 (カワヤツメ, シベリアヤツメ) の好中球の形態学的および細胞化学的特徴

Morphological and cytochemical characteristics of neutrophils from lampreys (Arctic lamprey Lethenteron camtschaticum and Siberian brook lamprey L.kessleri)

Creators Kondo Masakazu

Creators Yasumoto Shinya

Creators Matsubara Hajime

Two types of stratified (two-layer) granules (type 1, G-1； type 2, G-2) were observed in the neutrophils of lampreys, Lethenteron camtschaticum and L. kessleri. The G-1 consisted of chromophobic inner layer (L0) and outer layer (L1). On the other hand, the G-2 had chromatophilic L0 [eosinophilic and basophilic (metaazurophilic) in L. camtschaticum, basophilic (metaazurophilic) in L. kessleri] and chromophobic L1. Both lamprey species had same cytochemical characteristics. The L0 of G-1 showed positive reaction to alkaline phosphatase. Some lysozomal enzymes, such as acid phosphatase, β-glucuronidase, α-naphtyl acetate esterase and naphthol AS-D chloroacetate esterase were detected in the L0 of G-2. The L0 of G-2 was also Sudan black B positive. The neutrophils lacked α-naphtyl butyrate esterase and peroxidase.

[ISSN]0370-9361