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Cloning and Characterization of Bacterial Gene Involved in Chlorothalonil-Biotransformation

Journal of National Fisheries University Volume 56 Issue 1 Page 153-160
published_at 2007-11
56-1-153-160.pdf
[fulltext] 426 KB
Title
Cloning and Characterization of Bacterial Gene Involved in Chlorothalonil-Biotransformation
Creators Young-Mog Kim
Source Identifiers [PISSN] 0370-9361
Creator Keywords
chlorothalonil glutathione S-transferase biotransformation
A gene responsible for the chlorothalonil-biotransformation was cloned from the chromosomal DMA of Ochrobactrum anthropi SH35B, capable of efficiently dissipating the chlorothalonil. The gene encoding glutathione S-transferase (GST) of 0. anthropi SH35B was expressed in Escherichia coli, and the GST was subsequently purified by affinity chromatography. The fungicide chlorothalonil was rapidly transformed by the GST in the presence of glutathione. LC-MS analysis supported the formation of mono-, di- and tri-glutathione conjugates of chlorothalonil by the GST. The mono-glutathione conjugate was observed as an intermediate in the enzymatic reaction. The tri-glutathione conjugate has not been previously reported and seems to be the final metabolite in the biotransformation of chlorothalonil. It was here reported the glutathione-dependent biotransformation of chlorothalonil catalyzed by the bacterial GST.
Languages eng
Resource Type departmental bulletin paper
Publishers National Fisheries University
Date Issued 2007-11
File Version Version of Record
Access Rights open access
Relations
[ISSN]0370-9361