Morphological and Cytochemical Characteristics of Neutrophil from Japanese Amberjack, Seriola quinqueradiata

ブリの好中球の形態学的および細胞化学的特徴

Morphological and Cytochemical Characteristics of Neutrophil from Japanese Amberjack, Seriola quinqueradiata

Creators Kondo Masakazu

Creators Sakaguchi Takasuke

Creators Kanamaru Shunsuke

Creators Kashiwamura Naohiro

Creators Takahashi Yukinori

Morphological and cytochemical characteristics of neutrophil in Japanese amberjack, Seriola quinqueradiata were examined by light microscopy. The neutrophils were round to oval (7.0-11.0μm in diameter) and the nucleus round to lobule-shaped. Granules of the neutrophil were classified into three types； acidophilic granule (αG), chromophobic granule (βG), chromophobic granule (βG) and basophilic granule (γG). The αG was rod-shaped (0.5-1.0μm in length, 0.2μm in width) and stained with May-Grünwald (MG) stain. This granule was not observed in the preparations stained with Giemsa stain. The MG-Giemsa (MGG) staining pattern of the granule was influenced by pH and concentation of diluent of the staining solution. The βG was round to oval (≦0.5μm in diameter) and unstained by Romanovsky type stain (MG, Giemsa and MGG). This granule was alkaline phosphatase, peroxidase and sudan black B positive. The γG was round to oval (≦0.3μm in diameter) and stained with Giemsa and MGG, but not with MG. This granule was α-naphtyl acetate esterase positive. The Yasumoto body (Y-body) was also found in the neutrophil and toluidine blue positive.

[ISSN]0370-9361