Morphological and Cytochemical Characteristics of Neutrophil from Japanese Eel, Anguilla japonica

ウナギ好中球の形態学的および細胞化学的特徴

Morphological and Cytochemical Characteristics of Neutrophil from Japanese Eel, Anguilla japonica

Creators Kondo Masakazu

Creators Takahashi Yukinori

Morphological and cytochemical characteristics of neutrophil in Japanese eel, Anguilla japonica were examined by light microscopy. The neutrophils were round to oval (9.0-14.5μm in diameter) and the nucleus round to lobule-shaped. Granules of the neutrophil were classified into three types； acidophilic granule (αG), chromophobic granule (βG) and basophilic granule (γG). The αG was round to oval (0.3μm in diameter) or rod-shaped (≦1.0 μm in length) and stained with May-Grünwald (MG) stain. This granule was not observed in the preparations stained with Giemsa stain. The βG was round to oval (≦0.6μm in diameter) and unstained by Romanowsky type stain (MG,Giemsa and MG-Giemsa (MGG)). This granule was peroxidase positive. The γG was round to oval (≦0.3μm in diameter) and stained with Giemsa stain. Some enzymes, such as acid phosphatase, α-naphtyl acetate esterase and α-naphtyl butyrate esterase were detected in the γG. The Yasumoto body (Y-body) was also found in the neutrophil and toluidine blue positive.

[ISSN]0370-9361