Morphological characteristics of pseudoeosinophils and nigrosinophils in Kurloff's original papers

Kurloffの原著論文における偽好酸球とニグロシン好性細胞の形態学的特徴

Morphological characteristics of pseudoeosinophils and nigrosinophils in Kurloff's original papers

“Ehrlich’s ꞵ granules” refers to different granules identified in two types of leukocytes and is a term that has ambiguity. Among these, the ꞵ granules observed in the neutrophils of rabbits and guinea pigs stain with both acidic and basic dyes (amphophilic) and are now regarded as the granules of “pseudoeosinophils”, a term used for neutrophils in these species. However, it remains uncertain whether Kurloff (1889, 1892), who first introduced the term “pseudoeosinophils”, actually observed amphophilic granules in the cells. This study analysed Kurloff’s original papers and examined the morphological characteristics of the pseudoeosinophils he described. In addition, the papers include accounts of granular cells referred to as nigrosinophils. It also remains unclear which type of white blood cell, as recognised in modern haematology, corresponds to the cells. The results of this study suggest that the pseudoeosinophils observed by Kurloff refer to neutrophils in rabbits and guinea pigs, but their granules were not amphophilic and did not stain with basic dyes. Furthermore, the granules of pseudoeosinophils were assumed to exhibit staining characteristics similar to those of α granules, the specific granules of eosinophils, as demonstrated by triacid staining to confirm neutrophilic properties of granules and by staining with a triglycerine mixture that colours the α granules in the eosin staining properties. These staining results were considered to be due to the high temperature during the heat fixation of the specimens. The nigrosinophils were thought to correspond to basophils.

Creators Kondo Masakazu

Creators Yasumoto Shinya

[ISSN]0370-9361