Morphological and cytochemical characteristics of monocytes from inshore hagfish Eptatretus burgei
Journal of National Fisheries University Volume 72 Issue 3
Page 67-73
published_at 2024-03
Title
ヌタウナギ単球の形態学的および細胞化学的特徴
Morphological and cytochemical characteristics of monocytes from inshore hagfish Eptatretus burgei
Abstract
Monocytes were observed in the blood of inshore hagfish Eptatretus burgei. The monocytes were round or oval, high nucleus/cytoplasm ratio, and have several round or oval eosinophilic granules, which show dark red or blackish red color when stained with May-Grünwald (MG), Giemsa, and MG-Giemsa stain. The granules were positive for alkaline phosphatase, acid phosphatase, and α-naphthyl butyrate esterase. Also, the granules show metachromatic (reddish purple) with toluidine blue. However, ꞵ-glucuronidase, α-naphthyl acetate esterase, naphthol AS-D chloroacetate esterase and peroxidase were not detected in the monocytes, and negative for periodic acid Schiff reaction, alcian blue, Sudan black B, Sudan III, and oil red O. The monocytes engulfed many yeast particles (zymosan).
Source Identifiers
[PISSN] 0370-9361
Creator Keywords
hagfish
Eptatretus burgei
monocytes
morphology
cytochemistry
Languages
jpn
Resource Type
departmental bulletin paper
Publishers
National Fisheries University
Date Issued
2024-03
File Version
Version of Record
Access Rights
open access
Relations
[ISSN]0370-9361