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Neutrophil Granules of the Inshore Hagfish Eptatretus burgeri Infested with Parasites

Journal of National Fisheries University Volume 68 Issue 3 Page 83-91
published_at 2020-01
68-3-83-91.pdf
[fulltext] 2.77 MB
Title
寄生虫に感染したヌタウナギの好中球顆粒
Neutrophil Granules of the Inshore Hagfish Eptatretus burgeri Infested with Parasites
Creators Kondo Masakazu
Creators Yasumoto Shinya
Source Identifiers [PISSN] 0370-9361
Creator Keywords
granule neutrophil hagfish Eptatretus burgeri parasite
Four kinds of parasites, Ceratomyxa sp. (Myxozoa), Myxinidocotyle japonica (Monogenea; Mj), nematodes (Nematode; species unidentified) and Acanthochondria eptatreti (Copepoda; Ae), were observed in inshore hagfish Eptatretus burgeri. Here, we report the morphological and cytochemical characteristics of neutrophils from inshore hagfish infested with each parasite. In all infested fish, two types of stratified granules with two-layer structure [inner layer (L0) and outer layer (L1)] were observed in the neutrophils [type 1, G-1 (chromophobic L0 and basophilic L1); type 2, G-2 (basophilic L0 and chromophobic L1)]. The L0 of G-2 from all parasite-infested fish show positive reaction to acid phosphatase and toluidine blue. Except for Ae-infested fish, L0 of G-2 was also Sudan black B positive. Beta-glucuronidase was detected in the L0 of G-2 of nematodes-infested fish. The neutrophils from Mj-infested fish and nematodes-infested fish were naphthol AS-D chloroacetate esterase positive, but show different positive sites: Mj-infested fish, L0 of G-1; nematodes-infested fish, L0 of G-2.
Languages jpn
Resource Type departmental bulletin paper
Publishers National Fisheries University
Date Issued 2020-01
File Version Version of Record
Access Rights open access
Relations
[ISSN]0370-9361