Neutrophil Granules of Red Seabream Pagrus major Infected with Lamellodiscosis
Journal of National Fisheries University Volume 67 Issue 4
Page 269-272
published_at 2019-03
Title
ラメロジスカス症に罹患したマダイの好中球顆粒
Neutrophil Granules of Red Seabream Pagrus major Infected with Lamellodiscosis
Source Identifiers
[PISSN] 0370-9361
Creator Keywords
granule
neutrophil
Pagrus major
red seabream
lamellodiscosis
Two types of chromophobic granules [type 1, ordinary type (oβG-1^{Ld}); type 2, extraordinary type (eoβG-2^{Ld})] were observed in the neutrophils of red seabream Pagrus major infected with Lamellodiscus spp. The oβG-1^{Ld} showed similar morphology and cytochemistry to type 1 ordinary chromophobic granule (oβG-1) from non-infected fish: Chromophobic, simple morphology (without stratified structure), positive reaction to peroxidase (PO) and Sudan black B (SBB), and lack of lysozomal enzymes. The eoβG-2^{Ld} was stratified granule with two-layer structure [inner eosinophilic layer (L0) and outer chromophobic layer (L1)]. Several lysozomal enzymes [acid phosphatase (AcP), β-glucuronidase, α-naphtyl acetate esterase, α-naphtyl butyrate esterase, naphthol AS-D chloroacetate exterase (CAE)] were detected in the L0, however, AcP and CAE were localized in the small central area of L0. These findings indicate that the L0 of eoβG-2^{Ld} consists of L0-0 (inner part in L0; AcP and CAE positive) and L0-1 (outer part of L0-0). The L1 showed positive reaction to SBB and PO. Spot formation, a curious phenomenon appeared in PO-stained type 2 ordinary chromophobic granule (oβG-2; positive L1 and negative L0) from non-infected fish, was not observed in eoβG-2^{Ld}.
Languages
jpn
Resource Type
departmental bulletin paper
Publishers
National Fisheries University
Date Issued
2019-03
File Version
Version of Record
Access Rights
open access
Relations
[ISSN]0370-9361