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URIhttp://ypir.lib.yamaguchi-u.ac.jp/un/metadata/907
タイトル人工的悪性細胞の作製とその評価について
タイトル別表記Production of artificial malignant cells and their evaluation
作成者小林, 和香子
ガンフヤグ, エンフジャブフラン
中尾, 日香梨
作成者ヨミコバヤシ, ワカコ
ナカオ, ヒカリ
作成者別表記Kobayashi, Wakako
Gankhuyag, Enkhjavkhlan
Nakao, Hikari
著者キーワードEpithelial-mesenchymal transition (EMT)
transcription factor Snail
sialyl-lewis a (sLea)
資料タイプtext
ファイル形式application/pdf
内容記述(抄録等)The transcription factor Snail is involved in gastrula invasion, organogenesis, and cancer cell invasion/ metastasis during early embryogenesis. Snail also has the function of inducing epithelial-mesenchymal transition (EMT). The author introduced a Snail gene expression vector into human colon cancer cell line DLD-1 cells to prepare Snail overexpression cells (called DLD-1/Snail cells). DLD-1/Snail cells changed from epithelial-like morphology to mesenchymal cell morphology and decreased proteins levels such as epithelial makers and increased migration and invasion, inducing EMT. As a result of microarray analysis, the expression of α-1,3/4 fucosyltransferase (Fuc-TⅢ) was decreased in DLD-1/Snail cells compared with control cells. Fuc-TⅢ is a glycosyltransferase required to produce the cancer-related chain antigen sialyl-lewis a (sLea). It was a ligand that adhesive to vascular endothelial cells via E-selectin. It has been suggested that sLea is involved in cancer cell metastasis. However, decreased expression of Fuc-TⅢ in DLD-1/Snail cells reduced sLea antigens. The above results found that during EMT induction by Snail, the cells suppressed the fucosyltransferase expression and eliminated the cell surface's sLea antigen.
出版者宇部工業高等専門学校
出版者ヨミウベ コウギョウ コウトウ センモン ガッコウ
Nii資料タイプ紀要論文
本文言語jpn
ISSN0386-4359
NCIDAN00019833
掲載誌名宇部工業高等専門学校研究報告
67
開始ページ7
終了ページ12
発行日2021-03
著者版/出版社版出版社版
リポジトリIDUN20067000003
ファイルUN20067000003.pdf ( 2.2MB ) 公開日 2021-04-06
地域区分宇部工業高等専門学校