Cloning and Characterization of Bacterial Gene Involved in Chlorothalonil-Biotransformation

Cloning and Characterization of Bacterial Gene Involved in Chlorothalonil-Biotransformation

Creators Young-Mog Kim

A gene responsible for the chlorothalonil-biotransformation was cloned from the chromosomal DMA of Ochrobactrum anthropi SH35B, capable of efficiently dissipating the chlorothalonil. The gene encoding glutathione S-transferase (GST) of 0. anthropi SH35B was expressed in Escherichia coli, and the GST was subsequently purified by affinity chromatography. The fungicide chlorothalonil was rapidly transformed by the GST in the presence of glutathione. LC-MS analysis supported the formation of mono-, di- and tri-glutathione conjugates of chlorothalonil by the GST. The mono-glutathione conjugate was observed as an intermediate in the enzymatic reaction. The tri-glutathione conjugate has not been previously reported and seems to be the final metabolite in the biotransformation of chlorothalonil. It was here reported the glutathione-dependent biotransformation of chlorothalonil catalyzed by the bacterial GST.

[ISSN]0370-9361