コンテンツメニュー

Morphological and cytochemical characteristics of eosinophils from larva (ammocoetes) of far eastern brook lamprey Lethenteron reissneri

Journal of National Fisheries University Volume 70 Issue 4 Page 149-163
published_at 2022-03
70-4-149-163.pdf
[fulltext] 1.34 MB
Title
スナヤツメ幼生の好酸球の形態学的および細胞化学的特徴
Morphological and cytochemical characteristics of eosinophils from larva (ammocoetes) of far eastern brook lamprey Lethenteron reissneri
Abstract
Two types of stratified granules (two-layer) were observed in the eosinophils (eosinophil granule. EG: type 1, EG1; type 2, EG2) of larva (ammocoetes) of far eastern brook lamprey Lethenteron reissneri collected in a tributary of the Koyagawa River in Yamaguchi Prefecture. The EG1 consisted of inner eosinophilic layer (L0) and chromophobic outer layer (L1). Dark (low light transmittance) inclusion structure (IS), which was various size and morphology (round, oval, rod, or spindle), was observed in the L0 of many EG1 (only one IS in a EG1). The IS was found in the cytochemical staining preparation, but not in the preparation stained with May-Grunwald (MG), Giemsa and MG•Giemsa. Therefore, recognition of IS was affected with the eosin-stained L0 of EG1. The EG1 classified into three subtypes (EG1a, EG1b and EG1c) based on the optical artificial image (OAI) of IS in L0. The EG1a had no OAI (probably no IS). The OAI of both EG1b and EG1c were larger than IS. The former was round or oval chromophobic area (OAI-1), and latter was expanded and rugged (threedimensional) image (OAI-2; chromophobic; round, oval, or rod). The EG1a may be prototype of EG1. The EG1 showed no positive reaction by various cytochemical stains. The EG2 had chromophobic inner layer (L0) and basophilic (orthomethylenophilic) outer layer (L1). Some enzymes (alkaline phosphatase, acid phosphatase, ꞵ-glucuronidase, α-naphtyl acetate esterase, naphthol AS-D chloroacetate esterase) were detected in L0 of EG2. The eosinophils lacked α-naphtyl butyrate esterase and peroxidase.
Creators Kondo Masakazu
Creators Yasumoto Shinya
Source Identifiers
Creator Keywords
lamprey Lethenteron reissneri ammocoetes granulocyte morphology cytochemistry
Languages jpn
Resource Type departmental bulletin paper
Publishers National Fisheries University
Date Issued 2022-03
File Version Version of Record
Access Rights open access
Relations
[ISSN]0370-9361