Neutrophil Granules of Red Seabream Pagrus major Infected with Clavellotis dilatata

コブツキナガクビムシに寄生されたマダイの好中球顆粒

Neutrophil Granules of Red Seabream Pagrus major Infected with Clavellotis dilatata

Creators Kondo Masakazu

Creators Yasumoto Shinya

Two types of extraordinary chromophobic granules (type 1, eoG-1^{Cd}； type 2, eoG-2^{Cd}) were observed in the neutrophils of red seabream Pagrus major infected with Clavellotis dilatata (parasitic copepod). The eoG-1^{Cd} showed chromophobic, simple morphology (without stratified structure), peroxidase (PO) positive, Sudan black B (SBB) negative and lacked lysozomal enzymes. The eoG-2^{Cd} was stratified granule with two-layer structure [inner eosinophilic layer (L0) and outer chromophobic layer (L1)] and SBB negative. Alpha-naphtyl butyrate esterase and PO were detected in L0 and L1, respectively. Spot formation, a curious phenomenon appeared in PO-stained type 2 ordinary chromophobic granule (oG-2； positive L1 and negative L0) from non-infected fish, was not observed in the infected fish. Acid phosphatase (AcP), β-glucuronidase (β-Glu), α-naphtyl acetate esterase (α-NAE) and naphthol AS-D chloroacetate exterase (CAE) were detected in L0 of eoG-2^{Cd} as small central positive particles. These findings indicate that the L0 of eoG-2^{Cd} consists of L0-0 (inner part in L0； AcP, β-Glu, α-NAE and CAE positive) and L0-1 (outer part of L0-0).

[ISSN]0370-9361